FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Tsu-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Tsu-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33517221
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC3635771.0847468529685083TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG456600.065.0009546
GTATGCC470500.063.37074345
CGTATGC477800.062.46846844
CTAATCG479150.062.38030233
ATGCCGT476500.062.1614647
ACGTCTG501950.061.10896715
TCGTATG491050.060.55480643
GCACACG514900.059.66174311
TGCCGTC496050.059.47170648
GCCGTCT501800.058.41359349
CTCGTAT473150.058.38549842
CACACGT527900.058.22567412
ACTAATC517000.058.00293732
ACACGTC534450.057.5512913
CACTAAT536200.056.0891831
AGTCACT537050.055.9811928
CACGTCT553600.055.45185514
CGTCTGA553850.055.3763516
AGCACAC557300.055.27441410
GTCACTA545700.054.99087529
Produced by FastQC (version 0.11.5)