FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Tsu-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Tsu-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16530720
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1041170.6298394746266345TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG132650.060.2627746
GTATGCC135950.059.23762545
CGTATGC136550.059.00296444
TCGTATG144150.055.8678843
ATGCCGT143200.055.72525847
TGCCGTC144000.054.4434748
CACGTAG154400.054.1996531
GTCACGT157500.053.42158529
TCACGTA158000.053.1860730
CTCGTAT144150.052.8330642
ACGTCTG165800.052.31049715
GCCGTCT148300.051.37803649
AGTCACG165550.051.05647328
GCACACG181250.047.94875311
CACACGT181850.047.71341712
CGTAGAG178650.046.99930233
ACGTAGA178700.046.8686432
ACACGTC186950.046.33690613
CACGTCT191000.045.42712414
TCTCGTA168700.045.39351341
Produced by FastQC (version 0.11.5)