FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Tsu-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Tsu-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24710143
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG90250.038.39311215
GCACACG92150.037.56443411
GCGGGGT14500.036.6889850
CGGGGTG15950.035.54793551
CACACGT100950.034.324412
ATCGGGG32300.033.04899640
ACACGTC115200.030.0177813
CACGTCT122950.028.38127714
AGCACAC131500.027.1763310
CGTCTGA127750.027.12312116
GAGCACA136650.026.460999
TCGGAAG136400.025.3539033
GATCGGA145450.023.5338551
AATCGGG67500.023.17739939
ATCGGAA155200.022.37142
CAGTCAC165950.021.40674427
GGCGGGG24350.021.2727149
CTGAACT166900.021.01236519
CGGAAGA168050.020.9961264
TCCAGTC167750.020.84330425
Produced by FastQC (version 0.11.5)