FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Stw-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Stw-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29216361
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC3459251.1840112463013446TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG424650.065.7044346
CGTATGC437000.064.1519144
GTATGCC443600.063.47358345
ATGCCGT443700.062.36284347
TCGTATG452050.062.0935443
ACGTCTG490100.061.6953115
TGCCGTC448800.060.91332648
GCACACG498850.060.85986711
CACACGT505300.060.00712212
GCCGTCT448800.059.34584449
ACACGTC512100.059.2239813
CTCGTAT447650.058.95903442
ACTAATC454700.058.7686336
CCAACTA507150.058.24608633
CGTCTGA524700.057.6806316
AACTAAT507250.057.23402835
CACGTCT534900.056.6068914
AGCACAC551550.055.25532510
CAACTAA535850.054.96315434
GAGCACA557050.054.6818129
Produced by FastQC (version 0.11.5)