FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Stw-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Stw-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15115136
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC420370.2781119534749803TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC209950.063.84766833
CACCCGT216800.062.29850831
ACCCGTC218550.061.52741632
TCACCCG220850.061.17190630
GTCACCC223700.060.45514329
TATGCCG85150.059.14756446
ACGTCTG241250.057.9153915
CCGTCCA236600.055.96059434
GCACACG251600.055.75570311
CGTATGC86800.055.68435744
GTATGCC92500.055.54482345
CACACGT254700.055.1597212
ACACGTC260850.053.87264613
CGTCTGA262600.053.1535916
CACGTCT264200.053.00373514
ATGCCGT87300.052.1984347
TCGTATG88950.051.19064343
CAGTCAC268600.050.92278327
TCCAGTC274900.050.31612425
AGCACAC282250.049.77665310
Produced by FastQC (version 0.11.5)