FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Stw-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Stw-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24813050
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCC6750.011.9258351
ACGTCTG54550.011.22825815
GCCGGAT51900.011.1945191
CGCGTAT17350.011.0950161
GCCGAAT36750.011.0475341
GCCGGTT63700.010.9339821
CGGGCCT16800.010.6249181
CCCCGAT18300.010.5190441
CGGGCAT36600.010.5190441
GCCGATT47100.010.3290071
GCGGACT19450.010.256991
GCCAGCT84700.010.20653251
GCACACG61250.010.05732911
GCCCGAT21000.09.6665931
GGGGCGC6201.41535565E-89.59660329
CACACGT65900.09.56013512
GGCGCGC8453.6379788E-129.52643938
GCCCGCT15950.09.4356641
CCGGCAT52150.09.2616741
CGGGGCG3050.00599152469.1802571
Produced by FastQC (version 0.11.5)