FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.St-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.St-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23970479
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC1704610.7111288848253721TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG213000.063.13042446
CGTATGC221350.060.70151544
GTATGCC223050.060.4271745
ATGCCGT228600.058.62327647
TGCCGTC229150.057.7493248
ACGTCTG248600.057.49776515
TCGTATG232500.057.41423443
GCACACG259600.055.1297511
GCCGTCT236750.054.83109749
CACACGT267550.053.6095912
CTCGTAT237950.053.59873642
ACACGTC271150.052.9362213
CACGTCT281400.050.9827114
CGTCTGA282850.050.53553416
AGCACAC290350.049.72718410
GAGCACA300250.048.13689
TCTCGTA266000.047.82826641
TCGGAAG307500.046.6812823
CAGTCAC309650.045.57348327
CTGAACT324000.044.45143519
Produced by FastQC (version 0.11.5)