FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.St-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.St-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16383977
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG99300.047.2656215
GCGGGGT21850.046.13178652
CGGGGTG21750.044.4128953
GCACACG106550.044.1819911
CACACGT111600.042.3395312
ATCGGGG30500.042.11402542
ACACGTC111800.042.04477313
CACGTCT122000.038.67205414
ATGTCAG119100.038.4962834
CGTCTGA123800.038.1379316
AGCACAC127850.037.5617410
ACATGTC124000.037.22908432
GTCAGAA123150.036.88933636
CACATGT129850.035.3362131
TGTCAGA128700.035.32562335
GAGCACA137750.035.0937849
GTCACAT136150.033.9581829
TGCGGGG16850.033.44145651
TCACATG139500.033.09251830
CAGTCAC144350.032.41707627
Produced by FastQC (version 0.11.5)