FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.St-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.St-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17960383
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22050.032.69779646
GTCACGC29550.028.5443829
TCACGCC35650.024.15108530
CGTATGC30600.023.67602344
GTATGCC33850.022.12661445
CACGCCA39200.021.78536431
ACGTCTG41950.020.6911415
ATGCCGT34450.020.5221147
TCGTATG37550.019.75994743
GCACACG47350.018.84927211
CACACGT49700.017.53546712
ACACGTC52400.016.63187613
TATCTCG47250.016.44418139
TGCCGTC43750.015.83970148
ACGCCAA55700.015.457561532
CGCCAAT58200.014.61316833
AGTCACG61550.014.272758528
CTCGTAT51200.014.21852142
GCCGTCT49100.013.68609649
CGTCTGA66600.013.55845416
Produced by FastQC (version 0.11.5)