FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Shahdara_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Shahdara_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26056358
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT2786431.0693858289788618TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289420.11107461756550935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG315950.064.8476448
CGTATGC328050.062.5732446
GTATGCC328250.062.53511447
ATGCCGT335800.061.045649
ACGTCTG349400.060.4636715
TCGTATG341550.060.12048345
GCACACG356300.059.67699411
TGCCGTC343000.059.51928350
TATCTCG344500.058.5900541
CACACGT363350.058.54788612
GCCGTCT350100.057.9223651
CTCGTAT351100.057.47837444
ACACGTC372100.057.07683613
CACACTG365950.056.8293131
CGTCTGA387500.054.5548516
CACGTCT389850.054.2978214
AGCACAC397750.053.63480410
GAGCACA399350.053.47639
ACTGATA392150.053.15752834
ACACTGA395000.052.7650332
Produced by FastQC (version 0.11.5)