FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Shahdara_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Shahdara_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14831831
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17400.032.78678546
ACGTCTG41700.026.85840615
GCACACG42950.026.32155411
GTATGCC24450.025.3369845
CACACGT47800.024.01696612
CGTATGC24700.023.66355544
ACACGTC50900.022.1415913
ATGCCGT26100.019.712347
CGTCTGA61750.018.7043816
CACACAG61350.018.25565131
CACAGTG61050.018.05871233
CACGTCT63550.017.89934214
TCGTATG29400.017.6187443
ACACAGT64850.017.27038232
AGCACAC67800.017.19089310
GAGCACA71050.016.6520339
TGCCGTC32950.016.57027248
TCGGAAG79000.014.5328633
CTGAACT85900.013.649446519
TCCAGTC87400.013.45527925
Produced by FastQC (version 0.11.5)