FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Oy-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Oy-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24373106
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC940140.3857284336268016TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG297800.059.0344315
TATGCCG134100.058.3058846
GCACACG307700.057.5004511
ACGGTAG304100.056.63630732
CACACGT314850.056.07226612
AGTCACG307650.056.05149528
GTATGCC145700.055.8497745
GTCACGG311350.055.31760429
CGTATGC144150.054.89640444
ACACGTC322750.054.55879613
CGTCTGA333900.052.65196216
GGTAGCA325700.052.4077734
CACGTCT335700.052.3800714
AGCACAC344950.051.5559310
TCGTATG155800.051.0835443
GAGCACA354950.050.266699
ATGCCGT143700.049.076847
TCGGAAG361650.048.9859773
GTAGCAT344150.048.41847235
CGGTAGC357250.048.2301133
Produced by FastQC (version 0.11.5)