FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Oy-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Oy-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11919748
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT6460885.420315932853614TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT149890.12574930275371593TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG720300.067.2536948
CGTATGC730450.066.87018646
GTATGCC731400.066.8694747
ATGCCGT722750.066.56567449
TCGTATG735850.066.43178645
ACGTCTG794850.066.2265315
CTCGTAT717650.066.1901344
TGCCGTC714250.066.176950
GCCGTCT694750.065.9537651
GCACACG805800.065.4927411
CACACGT806800.065.3681812
CACAGTC797600.065.145831
ACACGTC811050.065.01214613
ACAGTCA799750.064.9750432
CACGTCT811200.064.89171614
TCTCGTA735650.064.7466143
CGTCTGA813300.064.693516
GTCACAG809550.064.3138629
CAGTCAC815000.064.1285727
TCCAGTC815200.064.0398525
Produced by FastQC (version 0.11.5)