FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Oy-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Oy-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15164660
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC171140.11285449195695782TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29500.043.89745346
GTATGCC37200.035.6579145
CGTATGC37650.035.045844
ACGTCTG46350.031.71574815
ATGCCGT42250.030.31893747
TCGTATG45100.029.64465743
TGCCGTC43500.029.36724548
GCACACG51350.028.08272711
CACTGAC55850.025.81874331
CTCGTAT48650.025.7551542
GCCGTCT47150.025.6834949
ACTGACC56050.025.47684332
CACACGT58400.024.99227112
ACACGTC61250.023.65793213
CACGTCT67600.021.95303214
AGTCACT70100.021.01963228
CGTCTGA69950.020.86525516
AGCACAC72500.020.51833210
GTCACTG76350.019.06976329
GAGCACA83850.018.5353019
Produced by FastQC (version 0.11.5)