FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.N13_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.N13_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23364894
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1921800.8225160362379559TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT300300.064.7427834
TATGCCG206800.063.5337548
CCGTACG306200.063.51814733
GTACGTA309850.062.98425735
TACGTAA318900.060.84602736
CGTATGC220500.060.80850246
GTATGCC226400.060.18229747
ACGTCTG332400.060.1445515
GCACACG335450.059.66119811
ACCGTAC327650.059.37053332
ATGCCGT214100.059.0461849
CACACGT341950.058.53735712
TCGTATG234700.057.59170545
ACGTAAT334400.057.54426237
ACACGTC348800.057.407713
TGCCGTC217150.055.76696450
CGTCTGA365300.054.81386616
CTCGTAT254300.054.6392744
CACGTCT367050.054.4574114
AGCACAC373000.053.8436810
Produced by FastQC (version 0.11.5)