FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.N13_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.N13_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12019763
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2149921.788654235528604TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC763120.6348877261556655TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG329850.066.688346
CGTATGC339000.065.9826944
GTATGCC342750.065.9449345
ATGCCGT317550.064.9839747
ACGTCTG498100.064.7718815
TGCCGTC306450.064.4025948
TCGTATG352250.064.3651643
GCACACG503900.064.1246511
CACACGT504250.064.0456912
ACACGTC507600.063.62274613
GTCACCT500900.063.33321429
CGTCTGA513600.062.8310116
CTCGTAT275200.062.7496942
CACGTCT515150.062.62131514
GCCGTCT295050.062.44262349
AGCACAC527000.061.40761610
AGTCACC522300.061.02643628
GAGCACA531950.060.952369
CAGTCAC524500.060.9241927
TCCAGTC525200.060.8896425
Produced by FastQC (version 0.11.5)