FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.N13_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.N13_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14339556
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43500.023.4943615
ACTTAGG44400.023.17537932
GCACACG45850.022.90102811
TATGCCG16600.021.50567246
CACACGT49650.021.43018212
CACTTAG48950.020.9496831
TCACTTA51750.019.81623830
ACACGTC54950.019.1721313
TTAGGCA54900.019.06161934
CGTATGC18750.019.03968844
GTATGCC21100.018.4120245
TAGGCAT55600.018.19214835
CACGTCT62500.016.96807714
AGTCACT63600.016.5643728
CGTCTGA64450.016.34607916
CTTAGGC67050.015.86849633
GTCACTT68100.015.26417429
AGCACAC72000.015.16689510
GAGCACA76900.014.7020549
TCGTATG26750.013.47641643
Produced by FastQC (version 0.11.5)