FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Mt-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Mt-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25319377
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2159120.8527539994368739TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG245600.063.22989748
CGTGGCC361900.062.32979633
GTATGCC258650.061.6499347
CGTATGC254650.061.01025446
GCCTTAT364300.060.8526437
ACGTGGC372100.060.82814432
ACGTCTG383650.060.64869715
CACGTGG379650.059.88593731
GTCACGT380700.059.7668529
GCACACG394150.059.140311
TCACGTG386850.058.79860730
CACACGT398850.058.45240412
ATGCCGT254400.058.41497849
AGTCACG392950.058.22453328
GGCCTTA387250.058.14101836
GTGGCCT388900.058.0024534
ACACGTC404900.057.5787813
TCGTATG261400.057.4798845
TATCTCG302550.057.12345541
TGCCGTC254900.056.51541550
Produced by FastQC (version 0.11.5)