FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Mt-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Mt-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20974866
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271240.12931667835208102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG301650.058.67563615
TATCGGG131200.058.36794739
TATCGCG70750.056.88952639
GCACACG314500.056.4239911
CACACGT317050.055.78250512
ACACGTC318750.055.57244513
CACTAGC315850.055.4716331
ACTAGCT316700.055.14592732
AGTCACT321500.054.8455328
CACGTCT326250.054.3587714
TCACTAG325150.053.9497330
TAGCTTA322000.053.90116534
GCGGGGT42800.053.88930550
CGTCTGA329850.053.72292316
CTTATCG224100.053.5845837
ATCGGGG86900.053.08313840
AGCACAC335900.052.96622510
GCTTATC310300.052.9559536
CGGGGTG47750.052.77400651
GTCACTA335950.052.3612329
Produced by FastQC (version 0.11.5)