FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Mt-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Mt-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17915743
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG46450.019.06350915
TATCGCG11500.017.34758639
GCACACG51950.017.11275311
CCGATGT50750.016.55149533
CACACGT54850.016.33564412
ACACGTC58000.015.50870813
TATGCCG15100.013.90705346
GATGTAT62250.013.88731935
CACGTCT69500.013.04316614
CGATGTA69200.012.74548834
CGTCTGA71650.012.70066516
ACCGATG71600.012.46491132
AGCACAC76200.012.08042410
GCCGGTT41200.011.8081221
GAGCACA86300.011.31638059
TGTATCG37150.011.21115137
ATGTATC78650.011.08058436
GTATCGC21900.011.02724438
GCCGAAT24700.010.7691121
GTATGCC23400.010.61948245
Produced by FastQC (version 0.11.5)