FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Mr-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Mr-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21205170
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2383781.1241503840808633TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC300850.065.7296133
TATGCCG282350.064.92908548
GTCCGCA306400.064.4133634
CCGCACA307100.063.22942436
GTATGCC293400.062.9608947
CGTATGC291350.062.89935746
TCCGCAC313400.062.8741335
ACGTCCG315950.062.71008732
ATGCCGT294300.061.97155449
CGCACAT311650.061.4189537
TCGTATG300100.060.7505145
ACGTCTG336350.060.74946215
GTCACGT331050.060.0930329
TGCCGTC301900.059.37970750
CTCGTAT303200.059.14819744
GCACACG347650.058.937211
GCCGTCT294250.058.59216351
AGTCACG341850.058.38905328
CACACGT353600.057.96526312
TCACGTC345950.057.50483730
Produced by FastQC (version 0.11.5)