FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Mr-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Mr-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22206430
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC16097087.248837386288566TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC418840.18861203714419653TruSeq Adapter, Index 9 (98% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267890.12063623013694681No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATGTCGTATGC261680.11783974281323023TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1862950.067.8046446
GTATGCC1871950.067.5197845
CGTATGC1874200.067.45740544
ATGCCGT1871500.067.3228247
TCGTATG1879450.067.1404743
ACGTCTG1925450.067.0322915
TGCCGTC1872650.067.0179548
GCCGTCT1862000.066.8148349
GCACACG1935250.066.7862811
CTCGTAT1803650.066.6925542
AGTCACG1920600.066.60232528
GTCACGA1918600.066.55865529
CACACGT1941850.066.530912
ACACGTC1944100.066.4699513
CGATCAG1919650.066.3818633
TCGGAAG1952650.066.29873
CACGTCT1953300.066.0962614
TCACGAT1932500.066.04368630
AGCACAC1959600.066.0004510
CGTCTGA1961200.065.7925416
Produced by FastQC (version 0.11.5)