FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_NutriNet.AtRep.Mr-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_NutriNet.AtRep.Mr-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26719559
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2590360.9694621082630892TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG317650.064.8202846
GTATGCC330050.062.7029945
CGTATGC331100.062.5781444
ATGCCGT334350.061.41517647
TCGTATG342400.060.4004743
TGCCGTC347350.058.7639748
CTCGTAT334400.057.857842
GCCGTCT351850.057.00773249
ACGTCTG401300.053.114715
TCACGAT383650.052.63826435
TCTCGTA365250.052.25229641
GTCACAT422700.050.0357629
CACGATC381050.049.5530536
CAGTCAC429500.049.4720227
ACACGTC432900.049.34338413
ATCACGA421550.049.28397834
CACACGT437200.048.78611412
GCACACG438200.048.7305111
CACGTCT438450.048.6222614
ATCTCGT387600.048.6162440
Produced by FastQC (version 0.11.5)