FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_untreated_2.00_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_untreated_2.00_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13035810
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT44250.042.7897735
ACTCCCG48850.039.54793532
CACGTCT53550.037.05736514
ACACGTC59250.033.7878313
AGTCACT58350.033.76876428
GTCACTC58350.033.7089129
CACACGT60450.033.4065912
ACGTCTG59850.033.21506515
CGTCTGA62650.031.89806616
CAGTCAC66950.030.21515827
CCGAATC59300.028.80189736
TCACTCC70850.028.0580730
GTCTGAA72550.027.83466717
TCGGAAG73500.027.33753
ATCGGAA73100.027.2478662
CACTCCC73400.026.98793431
CGAATCT35600.026.2494737
CTCCCGA74650.025.97342733
GCACACG81500.025.20769311
GATCGGA80950.024.780991
Produced by FastQC (version 0.11.5)