FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_untreated_2.00_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_untreated_2.00_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8611732
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC468390.5438975574251498TruSeq Adapter, Index 3 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT105980.12306467502704452No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG88130.10233713729131376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57550.065.80727446
GTATGCC59400.063.99267645
TCGTATG59700.063.4944843
CTCGTAT53550.063.1381642
CACGTCT65600.062.47403714
ACACGTC66750.061.39842213
ATGCCGT62850.060.4806547
ACGTCTG67600.060.47037515
TCACTCA66250.059.8532930
GCCGTCT65050.058.1661549
CGTATGC66050.058.07973544
TGCCGTC66300.057.33346648
CTGAACT72250.056.48130419
GAACTCC72650.055.88128721
GAGCACA74450.055.1896369
TCATTCA73300.053.19065534
GTCACTC74700.053.1767529
ACTCATT74500.053.0846232
AGTCACT75950.053.0388528
CACTCAT75350.052.6715831
Produced by FastQC (version 0.11.5)