FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_untreated_0.25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_untreated_0.25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12530681
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC301100.24029021247927387TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC46500.047.9480244
TATGCCG49750.044.81645246
TATCTCG49800.043.78502339
CTATAAT53900.042.79038233
TCGTATG53650.041.94869643
GTATGCC53400.041.49030345
CACTATA56950.040.49871431
CTCGTAT55100.039.3829942
ACTATAA60050.039.22397232
AGTCACT59250.038.8675428
CACGTCT62950.037.139314
ACGTCTG63100.036.9955515
TAATATC60750.036.29532636
GTCACTA64550.035.78483229
TATAATA64850.035.78119334
CACACGT66300.035.6853212
CGTCTGA66350.035.18326616
ACACGTC66650.035.1827213
CAGTCAC67950.035.02440327
ATGCCGT68500.032.4469847
Produced by FastQC (version 0.11.5)