FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_untreated_0.25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_untreated_0.25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9345595
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG27900.033.61911415
CACGTCT27650.033.54334614
ACACGTC30900.030.01548813
AGTCACT31350.028.69075228
ACTACAG32650.027.2268232
CACTACA32250.027.13042831
CAGTCAC38950.024.62021327
CACACGT39950.023.21610812
CGTCTGA41100.022.9068916
GTCTGAA43450.021.90938217
GTCACTA41300.021.18538529
CTGAACT44450.020.865119
TCTGAAC48200.019.75026318
TCACTAC45600.019.4946430
TCGGAAG47300.019.3892443
AGCACAC51100.018.8351810
TCCAGTC47900.018.77786825
TATGCCG11450.018.3415646
GCACACG52000.018.23986411
ATCGGAA50900.018.0868662
Produced by FastQC (version 0.11.5)