FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25047895
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5414082.1614910155124814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA679950.27145993705259464No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG431760.17237376633844878No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG392490.15669580218217938No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380330.1518411028152266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT86350.043.0570414
ACGTCTG88250.042.17093715
ACACGTC91900.041.36622213
CACACGT101950.037.3907112
CGTCTGA105800.035.1481416
TCGGAAG117100.035.125773
CTGAACT115200.032.25628319
ATCGGAA133400.031.4694982
GCACACG122700.031.12285211
GATCGGA132350.031.105421
CAGTCAC99000.030.48789427
AGCACAC133900.029.562310
AGGGGGG103700.027.8738731
GAACTCC135300.026.41720821
TGAACTC144750.025.2904920
CTCCAGT132500.024.40719624
CGGAAGA174050.024.2540574
AGAGCAC164000.024.2004388
CCAGTCA123900.024.15985526
AGTCACC69250.023.43663428
Produced by FastQC (version 0.11.5)