FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_2.00_R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_2.00_R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12300772
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC218520.17764738668434793TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39000.048.1939546
CGTATGC41950.045.30474544
GTATGCC44750.042.0007545
TCGTATG47100.040.3510443
CACGTCT51600.039.00089314
CTCGTAT46300.038.9307942
CTATACA51200.038.62169634
ACCTATA53600.036.56558632
CACCTAT53700.036.236831
ACACGTC56950.035.46012513
ACGTCTG57750.034.96861315
AGTCACC56850.034.78302428
CAGTCAC57000.034.56868727
CCTATAC59100.033.22205433
CACACGT61000.033.10607512
ATGCCGT59550.032.3856647
TATACAT62500.031.63902335
TCACCTA62500.031.13465930
TCTCGTA60950.029.97531741
GTCTGAA67500.029.81377217
Produced by FastQC (version 0.11.5)