FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_2.00_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_2.00_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8685025
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT160560.18486993416829545TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG24450.059.11854628
TATGCCG26350.054.1959848
GTATGCC26650.053.8482647
CACGTGA26950.053.7646231
GTCACGT27250.053.0442829
TCACGTG27750.052.0885330
TCGTATG27500.052.05508445
CACGTCT30150.050.9609214
ACGTGAT28600.050.78519432
ACACGTC30300.050.4779113
ACGTCTG31350.048.6753415
CTCGTAT24850.048.59205244
CGTATGC31250.045.8095346
ATGCCGT31700.045.15975649
GCCGTCT34800.040.4328551
CTGAACT38950.039.5364619
TGCCGTC37450.038.50640550
GAGCACA41150.037.7633639
TATCGTA5650.037.16831643
GATCCAA40000.036.22391536
Produced by FastQC (version 0.11.5)