FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_2.00_B2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_2.00_B2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11716464
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC146570.12509746968027213TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32350.042.4114646
CGTATGC35700.038.72546444
GTATGCC37550.037.0971845
CACGTCT42600.034.25951814
CTAATCG40800.034.226933
TCGTATG40650.034.0098243
AGTCACT41850.033.86993428
CTCGTAT36000.032.95802742
ACACGTC46550.031.87872513
ACGTCTG46000.031.80337515
CACACGT48700.030.68694712
CAGTCAC49700.028.44983527
ACTAATC50250.027.79019532
AATCGAT50100.027.03533435
GTCTGAA54750.026.97634517
CACTAAT54850.026.35288631
ATGCCGT52750.026.20862647
ATCGGAA55600.026.0654722
GTCACTA56950.025.44259529
CGTCTGA57850.025.40976716
Produced by FastQC (version 0.11.5)