FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_1.00_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_1.00_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10082222
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC119380.11840643858070175TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26150.039.21721344
TATGCCG27450.036.34076746
GTATGCC31500.031.55648245
TCGTATG32000.030.95372443
CACGTCT36100.028.50301214
CTCGTAT31450.028.3785142
CACACGT38550.027.32745612
ACACGTC38550.027.05482313
ACGTCTG39000.026.65277715
CGTCTGA39050.026.61864916
AGTCACC39900.026.40231928
CAGTCAC44250.024.12320527
TCGGAAG43200.023.740773
ATCGGAA45350.022.6160282
ATGCCGT45200.022.14721547
GTCTGAA47650.021.74099717
TCTCGTA41500.021.33744641
GCACACG56600.019.29270411
AGCACAC58200.019.18336510
GCCGTCT53850.018.84967449
Produced by FastQC (version 0.11.5)