FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_1.00_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_1.00_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8852577
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT131370.14839746663598635TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25500.041.3153548
CGTATGC27450.037.9974246
GTATGCC30850.034.15006647
TATCTCG29600.033.8169941
TCGTATG31950.033.5213145
CACGTCT36100.032.186914
CTCGTAT32100.031.0747644
ACACGTC39250.029.87125413
CACACGT40100.029.67446112
AGTCACA38500.029.6350228
GTCACAT38200.029.50161229
CGTCTGA40700.028.72105416
CCTTTAT39000.028.17852437
ACATTCC39650.028.1579332
CAGTCAC41500.027.83006127
ACGTCTG42400.027.40441315
TCTCGTA37050.026.73383543
ATCGGAA44300.026.1556682
TTATCTC38800.026.0691440
ATGCCGT40200.026.03336149
Produced by FastQC (version 0.11.5)