FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_0.50_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_0.50_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9567523
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT144620.15115720129442073TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG27100.046.23421528
CGTATGC29000.041.75969746
TATGCCG30350.040.24836748
GTCACGT34050.037.1060229
GTATGCC34100.035.5141147
TCGTATG35650.034.75527645
CACGTCT39900.034.1213714
ACGTCCG37150.033.7270632
CGCACAT38050.032.28544237
ACGTCTG43650.031.8314215
ACACGTC43300.031.68490813
GTCCGCA39500.031.5434734
TCACGTC40700.031.38721330
CACACGT44700.031.00589412
CTCGTAT36600.030.41030144
CCGCACA41450.029.72161736
TCCGCAC41800.029.6405235
CGTCTGA46950.029.29588916
ATGCCGT44050.027.88963149
CAGTCAC48050.027.45982227
Produced by FastQC (version 0.11.5)