FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_0.50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_0.50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7817059
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC110590.1414726433560243TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG20100.046.66494828
CGTATGC21050.044.39493644
TATGCCG20800.044.08801746
GTATGCC24450.038.50746545
CACGTAG24850.038.44904331
TCACGTA26050.036.54352630
GTCACGT26700.035.52303729
TCGTATG26200.035.26745643
CTCGTAT25800.032.96537842
CACGTCT30100.032.55694214
ACGTAGA29250.032.4259532
ACACGTC32450.030.30724113
ACGTCTG32900.029.78613915
CGTAGAG31850.029.66923733
CAGTCAC34450.028.64916227
ATGCCGT32650.028.086747
CACACGT35850.027.53054612
TAGAGAT35150.026.78456335
CGTCTGA37500.026.13220216
GTAGAGA37450.025.60686134
Produced by FastQC (version 0.11.5)