FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_0.25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_0.25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172412
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC163360.1780992829367019TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG28900.046.26142528
CGTATGC29550.044.4171844
TATGCCG30000.043.75282746
GTATGCC33300.040.15099345
GTCACGA33750.039.61370529
TCGTATG35200.037.38694843
CTCGTAT33550.034.63522342
CACGTCT40450.033.57137314
ACACGTC42150.032.4664713
ACGTCTG41950.032.37096415
CACACGT44750.030.8147812
CGTCTGA45200.029.96581316
ATGCCGT45250.029.70357747
CAGTCAC47800.028.99484627
CACGATC48400.028.12922531
GTCTGAA50900.027.3664717
GCCGTCT52000.026.1846749
TCACGAT53400.025.56094230
TCTCGTA45600.025.48271441
TCGGAAG53350.025.3251133
Produced by FastQC (version 0.11.5)