FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5.000_0.25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5.000_0.25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9570091
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21100.035.99782646
CACGTCT36250.034.1772814
ACGTCTG37000.033.20073315
AGTCACA37600.032.57768628
CGTATGC21950.032.53011344
ACACGTC39500.031.2767913
GTATGCC25050.030.04143145
CACACTT41550.029.14387731
CGTCTGA44550.027.57412116
CACACGT45750.027.30999812
CAGTCAC48000.027.1232527
TCGTATG25050.026.6876943
TCACACT47300.025.74900230
CTCGTAT25950.025.35706942
GTCACAC48550.024.8696629
GTCTGAA50850.024.50198717
TCGGAAG51600.023.4041773
ACACTTG53450.023.17916732
CTTGAAT52250.022.97495335
TTGAATC44600.022.83556436
Produced by FastQC (version 0.11.5)