FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_2.00_R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_2.00_R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9687575
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC326090.33660642627282883TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50700.054.12526346
CGTATGC52400.052.16782444
TCGTATG54550.050.17561743
GTATGCC55200.049.6484445
CTCGTAT52300.047.44758242
CACGTCT63450.045.56170714
ACGTCTG65400.043.8821315
AGTCACC65550.043.40796728
ACACGTC67350.042.8716513
ATGCCGT64450.042.84952547
CAGTCAC69450.040.66800727
CACACGT72150.039.9709712
CGTCTGA74850.038.57569516
GCCGTCT70700.038.51737249
GTCTGAA77650.037.45511617
TCTCGTA67400.037.07727441
TCGGAAG78850.036.5789223
ATCGGAA80350.036.0269282
TGCCGTC76800.035.9593848
TCCAGTC80250.035.58743325
Produced by FastQC (version 0.11.5)