FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_2.00_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_2.00_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7969966
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC607200.7618602137073107TruSeq Adapter, Index 7 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT365990.4592114947541808No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG147120.1845930083014156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG77100.064.690546
GTATGCC78800.063.95995345
CACGTCT118600.063.65330514
CTCGTAT79250.063.15433542
ACACGTC119950.062.90773413
ACGTCTG119900.062.90477815
TCGTATG76500.062.72525843
GAATATC112950.062.56167236
AATATCT102350.060.42364537
CTGAACT125250.060.05016319
CGGAATA122900.059.74603734
TATCTCG94400.059.46936839
GAGCACA128800.058.9385159
GTCACCG125050.058.63485329
GAACTCC127900.058.5596921
ATGCCGT82450.058.54012347
CGTATGC83100.058.33384344
GCCGTCT76300.056.97440749
TCCAGTC131600.056.59411225
AGTCACC132300.056.5856728
Produced by FastQC (version 0.11.5)