FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_2.00_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_2.00_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14579480
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC252210.17298970882363432TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47650.044.51376744
TATGCCG48800.043.39409346
CACCTAG56050.038.5260131
GTATGCC56100.038.18314745
TATCTCG52000.037.55784239
TCGTATG57100.037.26924543
ACCTAGC60200.036.0445532
CACGTCT63000.035.6653414
CTCGTAT56700.035.4330942
ACACGTC67350.033.56999213
ACGTCTG68050.033.12135315
AGTCACC67200.032.75863628
CACACGT69300.032.72650512
CAGTCAC70950.031.27384427
CTATCTC66000.029.80253638
ATGCCGT72600.029.3132247
CGTCTGA77650.029.20669716
ATCGGAA79900.028.3454842
GCTATCT70400.028.28749337
CCTAGCT77100.028.27991533
Produced by FastQC (version 0.11.5)