FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_1.00_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_1.00_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9386541
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT114510.12199382072693231TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22750.036.15477448
CGTATGC23350.034.9255846
TATCTCG26350.030.41710741
GTATGCC28250.028.99174147
CACGTCT31150.028.08911514
TCGTATG29400.027.97631345
CTCGTAT29050.027.71096644
AGTCACA32000.026.90522228
GTCACAC33350.025.71198329
ACGTCTG34750.025.2798815
CGTCTGA35150.024.59378616
CACACGT36100.024.5285412
ACACGTC36200.024.17073213
ACTGATA36900.023.33331334
CAGTCAC37950.023.05577327
TCGGAAG38300.022.6659413
TCTCGTA37000.022.04052243
ATGCCGT39450.021.204649
TATATCT40000.020.91225839
TCACACT41700.020.81521830
Produced by FastQC (version 0.11.5)