FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_1.00_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_1.00_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9626828
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC109050.11327718745987776TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG21800.043.66734328
TATGCCG23100.038.0321846
CGTATGC24400.036.57910544
GTATGCC28100.032.01175745
TCGTATG28100.031.76230643
CACGTCT32800.029.8764214
GTCACGG33150.029.13883229
CTCGTAT28400.029.08518442
ACACGTC34300.028.87612513
ACGTCTG37450.026.5406115
CACACGT38300.026.22585912
GGTAGCA38500.025.18083834
CAGTCAC39400.025.04939727
CGTCTGA42350.023.46979316
GTAGCAT43550.023.14514735
ATGCCGT38050.023.0891847
ACGGTAG42700.022.6217232
GTCTGAA44850.022.39565717
CGGTAGC46250.020.88545633
TCGGAAG46950.020.6526853
Produced by FastQC (version 0.11.5)