FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_0.50_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_0.50_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8756601
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC18000.034.02864544
TATGCCG18800.033.3255146
CACGTCT27150.029.77806514
GTATGCC21500.029.46586645
TCGTATG22600.027.2570243
ACGTCTG29650.027.03103315
ACACGTC30450.026.66582513
CACACGT32900.024.57383312
CTCGTAT22400.023.59367842
AGTCACC33550.023.15846828
CAGTCAC34750.022.3587527
CGTCTGA38350.020.8075716
GTCTGAA39000.020.72987717
TCGGAAG40200.019.7662183
ATGCCGT32800.019.31462947
ATCGGAA42500.018.944042
AGCACAC45650.018.55405410
GCACACG47350.017.14854611
CTGAACT46500.017.08528719
CACCCGT44600.016.87166431
Produced by FastQC (version 0.11.5)