FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_0.50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_0.50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100546
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT36850.034.00175514
CGTATGC20550.031.33933346
TATGCCG21100.031.02038448
AGTCACA40000.030.53618428
ACGTCTG42150.029.31100815
CACACGT43050.029.10515812
ACACGTC43150.029.03756313
GTCACAT41900.028.9011929
CGTCTGA45050.027.89003216
GTATGCC24900.027.12962747
CAGTCAC47550.026.57088727
TCGTATG26250.024.93361745
TCGGAAG51850.024.3705773
ATCGGAA52150.024.096382
GTCTGAA53800.023.87443717
CTCGTAT27800.023.79511844
CACATGT53650.022.8324231
GTCAGAA54200.022.40722736
GCACACG58550.021.9382211
GATCGGA58100.021.5702481
Produced by FastQC (version 0.11.5)