FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_0.25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_0.25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8023076
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC95300.1187823722472528TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG17900.042.62354328
CGTATGC18600.039.70501344
TATGCCG18800.039.28286746
TATCTCG21900.033.5614139
GTATGCC22100.033.4168945
TCGTATG22300.033.11718843
CGCCAAT24450.031.7781333
CACGTCT26050.030.49768814
CTCGTAT24200.030.22762942
ACGTCTG26950.029.21930115
GCCAATA27100.028.79982234
ACACGTC28250.028.24653413
ATGCCGT27700.026.40858347
CAGTCAC35250.024.5235827
CACACGT34400.023.60360512
CACGCCA33350.023.50749631
CCAATAT33650.023.2982135
ACGCCAA34550.023.09622432
GCCGTCT34100.022.27354449
ATATCTC34300.022.04052538
Produced by FastQC (version 0.11.5)