FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2.500_0.25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2.500_0.25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10420063
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19250.036.00134746
CGTATGC20200.032.74828344
CACGTCT27950.029.80176414
GTATGCC23800.028.82419645
ACACAGT30650.026.6055532
TCGTATG23600.026.54722243
ACACGTC30900.026.39042313
AGTCACA30450.026.32042528
ACGTCTG31350.026.23476215
CAGTCAC39450.022.88850627
ATGCCGT31300.021.8061447
CACACGT38850.021.35051312
ACAGTGA39600.019.88558434
GTCACAC40100.019.81229
CTCGTAT21950.019.77257242
GTCGTAT10050.019.1545842
GCCAGTA30450.018.856351
GTCTGAA44000.018.77171917
CACACAG44950.018.37507431
AGCACAC50650.017.6203210
Produced by FastQC (version 0.11.5)