FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_2.00_R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_2.00_R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8427422
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC802900.9527231459395293TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATGTCGTATGC145820.17303037631199672TruSeq Adapter, Index 7 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT97880.11614465254024303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG123500.064.7039746
CGTATGC124850.064.14146444
GTATGCC126100.063.9501245
TCGTATG124000.063.16946843
CACGTCT142950.063.068514
ACACGTC145500.062.17965713
ACGTCTG145450.062.05666415
CTCGTAT102050.062.0089342
ATGCCGT130950.060.7826447
GCCGTCT128350.059.2599249
CTGAACT153200.058.5975419
TGCCGTC134500.057.92953548
CGTCTGA157300.057.3594516
TCCAGTC154500.057.28898625
TCGGAAG157900.057.2604683
GAGCACA161000.056.4111379
AGTCACC160650.056.31583828
GAACTCC159000.056.04179821
CAGGCGA155600.055.89463834
TCTGAAC161550.055.8721218
Produced by FastQC (version 0.11.5)