FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_2.00_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_2.00_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8799223
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT108270.12304495521934153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT97900.063.02642414
ACACGTC98150.062.93720213
ACGTCTG100600.061.5088115
CTGAACT105500.058.4526219
CATGGCA103850.057.3939534
GAGCACA108800.057.1310279
GAACTCC108550.056.5845521
TCCAGTC114900.052.93955225
TCGGAAG117050.052.9006843
AGTCACC117650.052.38633328
TCTGAAC118800.052.17382418
ACTCCAG118100.051.89037323
CGTCTGA121350.051.02007716
GCACACG121700.050.95990811
AACTCCA121450.050.51669722
GTCACCA123200.048.9755829
CAGTCAC127300.048.8000827
CACACGT127800.048.5001612
GTCTGAA127650.048.4743517
TCACCAT124400.048.41874330
Produced by FastQC (version 0.11.5)