FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_2.00_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_2.00_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8378159
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC917081.0946080159137586TruSeq Adapter, Index 9 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT244490.2918182860936394No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG101730.12142285673976826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG119500.065.6689846
GTATGCC124650.063.8820945
CGTATGC121950.063.83228344
TATCTCG125300.063.1577339
TCGTATG119750.063.0758843
CTCGTAT117300.062.96066342
CACGTCT161100.062.95806514
ACACGTC163600.062.18852613
ACGTCTG162650.062.16443615
ATGCCGT125150.061.50167547
CATTTTA162600.060.72026434
TGCCGTC126100.059.53944848
GCCGTCT121300.059.21191849
CTGAACT175200.057.7310819
TCCAGTC173750.057.50786625
AGTCACC174150.057.49635728
GAACTCC174800.057.3626421
GAGCACA178450.057.1121569
ACTCCAG176000.057.05107523
ATTTTAT172050.056.97898535
Produced by FastQC (version 0.11.5)