FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_1.00_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_1.00_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225902
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT194230.23612000240216818TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG27750.055.87119328
TATGCCG29650.050.7604348
GTCACGA32650.047.59372329
CGTATGC32150.047.46619446
GTATGCC32450.046.7037947
CTCGTAT33400.043.90748244
TCGTATG35000.043.900345
TATCTCG33900.043.0523441
TCACGAG37600.041.3280630
CACGTCT38500.040.99803514
ACACGTC40150.039.7492713
ACGTCTG40400.039.0699115
CACGAGT40200.038.39391331
ACGAGTG40600.038.01564832
ATGCCGT40250.037.39246449
CAGTCAC44200.036.4235927
GGATATC43150.034.06661238
CACACGT47250.033.48048812
GTCTGAA47400.033.4477617
GATATCT44250.033.0615739
Produced by FastQC (version 0.11.5)